Additional file 1.

Gq/11/14 and Gagus staining in vallate taste buds in a TrpM5-GFP mouse. Single confocal image planes from the image shown in Fig. 3A; TrpM5-GFP (B, green), Gagus (D, blue) and Gq/11/14 (F, red), respectively. Co-localization in single plane images of TrpM5-GFP and Gagus (A), TrpM5-GFP and Gq/11/14 (C), Gagus and Gq/11/14 (E) in the vallate papilla. Panel G shows the the z-stack of the combined Gαgus and Gq/11/14 images equivalent to text Fig. 3A. TrpM5 is a marker of Type II taste cells. Gq/11/14 stains about half (14 of 27) of the TrpM5-GFP cells. Usually the Gq/11/14 immunoreactive cells are different from those positive for Gagus. Scale bar 20 μM.

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Tizzano et al. BMC Neuroscience 2008 9:110   doi:10.1186/1471-2202-9-110