Gα14 subunit expression in vallate papilla. Using Gαq-null mice and Gα11-specific anitserum, we show that the immunoreactivity in taste buds revealed by Gq/11/14 antiserum must be due to Gα14. (A) Gq/11/14 immunoreactivity in vallate papilla of (A) WT and (A') Gαq null mice. The continued immunoreactivity for Gq/11/14 in Gαq-null mice demonstrates that members of the Gαq-family other than Gαq are responsible for most of the Gq/11/14 antibody immunoreactivity. (B) Gαgus immunoreactivity in (B) WT and (B') Gαq null mice. Continued immunoreactivity for Gαgus shows that other Gα expression is not altered in the Gαq null animals. Scale bars = 40 μM for A-D. C-F: Micrographs of immunostaining of TrpM5-GFP mice with an antiserum specific for Gα11 (C, C', E) or the broad Gq class antiserum (Gq/11/14) which reacts with Gαq, Gα11 and Gα14 (D, D', F). C, C': Section through taste buds of the vallate papilla stained with Gα11-specific antiserum (red). C. shows the combined image of TrpM5-GFP (green) and Gα11 (red); C' shows only the red channel of this same image. No specific Gα11 staining is evident. This exposure is matched to that of panel E. E. Gα11 staining of the cerebellum showing evident immunoreactivity of the molecular layer corresponding to the demonstrated presence of Gα11 in Purkinje cell dendrites[35,56]. D, D': Sections through taste buds of the vallate papilla stained with Gq/11/14 antiserum (red). D shows the combined image of TrpM5-GFP (green) and Gq/11/14 (red); D' shows only the red channel of this same image. Many taste cells in each taste bud show clear membrane-associated immunoreactivity similar to that shown in Fig. 3. F. Gq/11/14 staining of the cerebellum showing evident immunoreactivity of the molecular layer corresponding to the demonstrated presence of Gα11 in Purkinje cell dendrites [35,56]. Since Purkinje cells express both Gαq and Gα11, and higher levels of Gαq than Gα11, immunoreactivity with the broad Gq/11/14 antiserum is much greater than that obtained with the specific Gα11 antiserum. Exposure for this panel is 20% of that for panels D, D'. Scale bar in F (50 μm) also applies to panels C, D & E.
Tizzano et al. BMC Neuroscience 2008 9:110 doi:10.1186/1471-2202-9-110