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This article is part of the supplement: Annual Meeting of the Study Group Neurochemistry. International Conference of the Gesellschaft für Biochemie und Molekularbiologie 2006 (GBM 2006): Molecular pathways in health and disease of the nervous system

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GABAC receptor rho subunits interact with PNUTS, a targeting subunit of protein phosphatase 1

Ralf Enz1*, Heinrich Sticht1 and Johann Helmut Brandstätter12

  • * Corresponding author: Ralf Enz

Author Affiliations

1 Institut für Biochemie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany

2 Institut für Biologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany

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BMC Neuroscience 2007, 8(Suppl 1):P32  doi:10.1186/1471-2202-8-S1-P32

The electronic version of this article is the complete one and can be found online at:

Published:23 March 2007

© 2007 Enz et al; licensee BioMed Central Ltd.

Poster presentation

Synaptic signal transduction depends on a precise regulation of neurotransmitter receptors by kinases and phosphatases. However, molecular mechanisms that specify the interaction between these proteins are largely unknown. Performing yeast two-hybrid screens using intracellular domains of GABAC receptor rho subunits as baits, we identified PNUTS, a nuclear targeting subunit of protein phosphatase 1 (PP1). PP1 contains several catalytic domains designated as PP1alpha, PP1beta/delta and PP1gamma1/2 that bind to a short motif in PNUTS composed of five amino acids. PNUTS interacted with one intracellular domain of the rho1/3 and with two intracellular regions of rho2. The rho interacting domains of PNUTS were different from its PP1 binding motif and consequently, PNUTS formed a ternary complex together with PP1gamma1 and rho2. Furthermore, we analyzed the structure of PNUTS in contact with the enzyme by homology-based molecular modeling. In the retina, immunohistochemical analys is showed expression of PNUTS in nuclei of cell bodies located in the inner nuclear layer and in the ganglion cell layer. In addition, Western blotting detected PNUTS outside the nucleus in cytosolic and membrane protein fractions of retinal cells. Specific label for the PNUTS binding partners PP1alpha, PP1beta, PP1gamma1 and GABAC receptors was mainly observed as clusters of immunofluorescent puncta in the synaptic layers of the retina. Finally, we show that PP1gamma1 and GABAC receptors are co-expressed in axon terminals of rod bipolar cells that express PNUTS. Based on our data, we suggest that PNUTS acts as a temporary bridge between GABAC receptors and catalytic domains of PP1.