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Grading of neurological severity score of rats. |
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| Behaviors |
Points |
||
|
|
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| Hemiplegia: inability resist to forced changes in position |
1 |
||
| Flexion in hindlimb when raised by tail |
1 |
||
| Inability to walk straight when placed on floor |
1 |
||
| Inability to walk |
1 |
||
| Reflex |
Loss of righting reflex for |
20 minutes |
1 |
| 40 minutes |
1 |
||
| 60 minutes |
1 |
||
| Limb reflexes: loss of placing Reflexes |
Left forelimb |
1 |
|
| Left hindlimb |
1 |
||
| Right forelimb |
1 |
||
| Right hindlimb |
1 |
||
| Clinical grade |
Loss of seeking behavior |
1 |
|
| Prostration |
1 |
||
| Inability to exit from a circle (50 cm in diameter) when left in the center for Functioning test: failure in beam balancing task (1 cm wide) |
20 minutes |
1 |
|
| 40 minutes |
1 |
||
| 60 minutes |
1 |
||
| Balances with steady posture, paws on top of beam |
1 |
||
| Grasps sides of beam and/or has shaky movement |
1 |
||
| One or more paws slip off beam |
1 |
||
| Attempt to balance on beam but falls off |
1 |
||
| Drapes over beam and/or hangs on beam and falls off |
1 |
||
| Falls off beam with no attempt to balance or hang on |
1 |
||
| Failure in beam walking task |
2.5-cm wide |
1 |
|
| 5.0-cm wide |
1 |
||
| 8.0-cm wide |
1 |
||
| Sum |
25 |
||
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The table summarizes the criteria for scoring of rats. The NSS correlates directly with the deterioration of observable neurological status so that a lower score represents nearly intact neurological status and a higher score indicates severe neurological dysfunction caused by injury. | |||
Fei et al. BMC Neuroscience 2007 8:96 doi:10.1186/1471-2202-8-96 |
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