Additional file 1.

Auto-fluorescent particles fluoresce in both color channels in the GLL. Visualizing unlabeled tissue shows the presence of auto-fluorescent particles in the GLL (A). These particles auto-fluoresce under the wavelengths of light used to identify the secondary antibody for (B) BrdU (emits red, excited by 594 nm) and (C) NC, CR, TH, GABA, and CB (emits green, excited by 488 nm), resulting in their appearance as double labeled cells. Scale bars, 74 μm. GLL, glomerular layer; BrdU, bromodeoxyuridine; NC, N-copine; CR, calretinin; TH, tyrosine-hydroxylase; CB, calbindin; MCL, mitral cell layer; EPL, external plexiform layer GCL, granule cell layer.

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Bagley et al. BMC Neuroscience 2007 8:92   doi:10.1186/1471-2202-8-92