Adult neurogenesis and specific replacement of interneuron subtypes in the mouse main olfactory bulb

doi:10.1186/1471-2202-8-92

BMC Neuroscience

Volume 8

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Research article

Adult neurogenesis and specific replacement of interneuron subtypes in the mouse main olfactory bulb

Joshua Bagley²^,5 , Greg LaRocca¹ , Daniel A Jimenez and Nathaniel N Urban¹^,2^,3^,4

¹Department of Biological Science, Carnegie Mellon University, Pittsburgh, PA 15213, USA

²Department of Neuroscience University of Pittsburgh, Pittsburgh, PA 15260, USA

³Center for the Neural Basis of Cognition, Pittsburgh, PA 15213, USA

⁴Center for Neuroscience at the University of Pittsburgh, Pittsburgh, PA 15213, USA

⁵National Institutes of Health/NINDS, 35 Convent Drive, Bldg.35/Rm. #A108, Bethesda, MD 20892-3703, USA

author email corresponding author email

BMC Neuroscience 2007, 8:92doi:10.1186/1471-2202-8-92


Published:	9 November 2007

Additional files

Additional file 1:

Auto-fluorescent particles fluoresce in both color channels in the GLL. Visualizing unlabeled tissue shows the presence of auto-fluorescent particles in the GLL (A). These particles auto-fluoresce under the wavelengths of light used to identify the secondary antibody for (B) BrdU (emits red, excited by 594 nm) and (C) NC, CR, TH, GABA, and CB (emits green, excited by 488 nm), resulting in their appearance as double labeled cells. Scale bars, 74 μm. GLL, glomerular layer; BrdU, bromodeoxyuridine; NC, N-copine; CR, calretinin; TH, tyrosine-hydroxylase; CB, calbindin; MCL, mitral cell layer; EPL, external plexiform layer GCL, granule cell layer.

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Additional file 2:

Confocal imaging taken of sections double labeled for BrdU and GABA (top left) N-copine (bottom left) and calretinin (right). Images show projections and also Z-zxis sections through the cell of interest (indicated by the intersecting lines). In all cases when analyzing double labeling we required that the label in the two colors be overlapping in all three dimensions.

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