Fibroblast- like cells from the olfactory system and sciatic nerve express calponin. Neonatal olfactory bulb (A, B) and the sciatic nerve (C, D) were dissociated to obtain purified fibroblast-like cell cultures respectively. A, C) Purified fibroblast-like cultures from the olfactory bulb (A) and sciatic nerve (C) expressed calponin immunoreactivity (green) with typical fibrous appearance. B, D) Purified fibroblast-like cells immunolabeled for the connective tissue markers Thy1.1 (red) and α-SMA (green) in both olfactory bulb (B) and sciatic nerve (D). Colocalisation of both markers with calponin was shown for sciatic nerve fibroblasts. All images were obtained using a fluorescent microscope.
Tomé et al. BMC Neuroscience 2007 8:74 doi:10.1186/1471-2202-8-74