Purity evaluation of isolated mitochondria by WB. Equal amounts of protein (20 μg for cytochrome C and nucleolin, 5 μg for tubulin) were loaded onto an 8–16% SDS-PAGE and analyzed by WB with indicated antibodies against marker proteins from mitochondria, cytosol, or nucleus. Antibodies against α-tubulin and nucleolin were used as markers for cytosolic and nuclear fractions, respectively. Antibodies directed against cytochrome C were used as markers for mitochondrial fraction.
Jin et al. BMC Neuroscience 2007 8:67 doi:10.1186/1471-2202-8-67