Figure 4.

Representative photomicrographs of immunohistochemistry for tyrosine hydroxylase (TH) labeled neurons in AVPV of 150 day-old Long-Evans rats fed either an isoflavone-free diet (Phyto-free) or an isoflavone-rich diet (Phyto-600) from conception until time tissue collected. TH-immunoreactive(ir) cells were visualized with diaminobenzadine (DAB) and seen as brown cytoplasma staining. Sections were counterstained with hematoxylin (blue). Positive control of TH staining is shown in Phyto-free fed female AVPV (A). In males, no significant differences in the number of TH-ir neurons were observed between Phyto-600 (C) and Phyto-free AVPV (B). The TH-ir cells in the boxed regions were indicated (*) at high magnification in the up-left corner of B and C. Bar = 25 μm for all the photomicrographs.