Figure 12.

Various 20-mers with thymine modifications were tested for inhibitory activity in the assay outlined in Figure 1. HDT, 20-mer with all T's; HDG, 20-mer with all G's; HDG 20/7, 20-mer with every 7^th nucleotide replaced with T; HDG 20/4, 20-mer with every 4^th nucleotidereplaced with T HDG 20/3, 20-mer with every 3^rd nucleotide replaced with T; HDG 20/2, 20-mer with every other nucleotide replaced with T. 0 hour, reaction stopped at zero time point; 24 hour, reaction lacking oligonucleotide and reaction stopped at 24 hours; Congo Red, incubation with Congo Red (10 μM) for 24 hours.