Table 1

Primary antibodies and lectins

Antigen

Host

Dilution

Source (catalog #; clone #)

Reference


Syntaxin 1

Mouse

1:500–1:1000

Sigma Chemical Company, St. Louis, MO (S0664; clone HPC-1)

57

Syntaxin 2

Rabbit

1:100–1:200

Synaptic Systems, Göttingen, Germany (110-022)

108

Syntaxin 3

Rabbit

1:750–1:1000

Novus Biologicals, Littleton, CO (AB4113)

5,109

Syntaxin 4

Rabbit

1:100–1:200

Chemicon International, Temecula, CA (AB5330)

95,110

Bassoon

Mouse

1:500

Stressgen Biotechnologies, Victoria, British Columbia, Canada (VAM-PS003; clone SAP7F407)

111

Calbindin

Mouse

1:300

Sigma Chemical Company, St. Louis, MO (C9848; clone CB955)

---

CD15

Mouse (IgM)

1:50

BD Biosciences Pharmingen, San Diego, CA (555400; cloneHI98)

35

Goα

Mouse

1:500–1:1,000

Chemicon International, Temecula, CA (MAB3073; clone 2A)

112

Glutamic Acid Decarboxylase, 65 kDa (GAD-65)

Mouse

1:500–1:1000

Chemicon International, Temecula, CA (MAB351; clone GAD-6)

113

Glycine transporter 1 (GlyT1)

Goat

1:2000

Chemicon International, Temecula, CA (AB1771)

---

Glutamine synthetase

Mouse

1:1000

Chemicon International, Temecula, CA (MAB302; clone GS6)

114

Kinesin II

Mouse

1:50

Covance Inc., Princeton, NJ (MMS198P; clone K2.4)

115

Microtubule Associated Protein 1 (MAP-1)

Mouse

1:300–1:500

Sigma Chemical Company, St. Louis, MO (M4278; clone HM-1)

116

Peanut Agglutinin (PNA)

---

1:10

Molecular Probes, Eugene OR

117

Postsynaptic density protein-95 Kda (PSD-95)

Mouse

1:100

Upstate Biotechnologies, Lake Placid, NY (05–494; clone K28/43)

---

Synaptic vesicle protein 2 (SV2)

Mouse

1:20–1:100

Dr. K Buckley, Harvard Medical School, Boston, MA

118

Synapsin I

Mouse

1:500–1:1000

Chemicon International, Temecula, CA (MAB355)

119

Tyrosine hydroxylase (TH)

Mouse

1:500

Chemicon International, Temecula, CA (MAB318; clone LNC1)

---

Vesicular glutamate transporter 1 (VGLUT1)

Guinea pig

1:2500–1:5000

Chemicon International, Temecula, CA (AB5905)

28

Vesicular glutamate transporter 3 (VGLUT3)

Guinea pig

1:2500

Chemicon International, Temecula, CA (AB5421)

26


Sherry et al. BMC Neuroscience 2006 7:54   doi:10.1186/1471-2202-7-54

Open Data