Figure 9.

Analysis of traced TGNs for TTX-sensitivity of sodium currents and IB4 binding. (A) Exemplary sodium inward currents of nasal and cutaneous TGNs without (black) and in presence of 500 nM TTX (red). Vertical bars: 2 nA; Horizontal bars 10 ms (B) Mean amplitude of sodium inward currents in presence of TTX. Sodium currents were normalized to currents without TTX (= 100%). Error bars indicate standard deviation. (C) IB4 staining of TGN cell culture following in vivo infection (intranasal inoculation of PrV-614). White arrowhead: IB4 negative traced neuron, Blue arrowheads: IB4 positive traced neurons, Bar: 10 μm (D) Fractions of IB4 binding neurons within the groups of control (n = 72), nasal (n = 19), and cutaneous (n = 36) TGNs.