Figure 1.

Genomic map of the PrV-Bartha strains used in this study. (A) shows a schematic diagram of the wildtype PrV genome with the unique long (U_L), unique short (U_S) and inverted repeat (IR: internal repeat; TR: terminal repeat) regions and a BamHI restriction fragment map. Fragments are numbered according to their size. In (B) the region used for insertion of marker gene cassettes is enlarged, relevant restriction sites are indicated and open reading frames are shown by rectangles. The region enlarged contains genes encoding a protein kinase (PK; US3) and glycoproteins (g)G (US4), gD (US6), gI (US7) and gE (US8), as well as the 11 K (US9) and part of the 28 K (US2) protein. The hatched box indicates the large deletion of sequences in PrV-strain Bartha compared to the laboratory strain Kaplan. PrV-614 comprises the mRFP1 under control of the CMV ie promoter/enhancer (CMV ie1) [16] inserted into the PstI site located in the gG gene. PrV-Cam expresses the Yellow Cameleon under the same promoter, substituting a short BamHI-fragment in gG, while the β-galactosidase sequences in PrV-B80 are fused to the first 8 codons of the gG open reading frame and are under control of the PrV gG gene promoter [18].