Table 3

Effects of inhibitors of caspases 3, 8 and 9 against toxicity and ROS accumulation after serum deprivation in enriched hippocampal neuronal cultures, as estimated by MTT and DCF assays, respectively.

Drugs

MTT values (% of control)

DCF values (% of control)


Control

100 ± 4

100 ± 6

+ Inhibitor of caspase 3 (DEVDO-CHO, 5 μM)

171 ± 12 *

85 ± 4

+ Inhibitor of caspase 8 (IETD-CHO 5 μM)

141 ± 9 *

96 ± 5

+ Inhibitor of caspase 9 (LEHD-CHO, 5 μM)

135 ± 10 *

89 ± 6

+ Clozapine (1 μM)

140 ± 8 *

ND


Data represent mean ± SEM of at least three separate experiments. *p < 0.01 compared to vehicle-treated cells. ND: not determined

Bastianetto et al. BMC Neuroscience 2006 7:28   doi:10.1186/1471-2202-7-28

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