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Efficacy of select estrogens within CEE to sustain the intracellular ATP level following exposure to β-amyloid25–35 in basal forebrain neurons. Neuronal cultures were pretreated with indicated estrogens for 4 days followed by a 24 hr exposure to 8 μg/ml β-amyloid25–35. The culture medium was replaced and cultures were allowed to incubate for an additional 24 hr followed by determination of the intracellular ATP level. Data are represented as mean ± SEM (n = 7 – 24 from 3 separate experiments). ** P < 0.01 and *** P < 0.001 compared to β-amyloid25–35 alone-treated cultures. |
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| Treatment (pg/ml) |
ATP Level (% of β-amyloid25–35 alone group) |
|
|
|
| Control |
203.37 ± 11.67*** |
| β-amyloid25–35 alone |
100.00 ± 2.07 |
| 17α-estradiol (10) |
103.83 ± 3.76 |
| 17β-estradiol (10) |
131.32 ± 4.56** |
| Equilin (1000) |
114.58 ± 3.56 |
| Equilenin (300) |
96.44 ± 4.07 |
| 17α-dihydroequilin (1000) |
110.00 ± 2.75 |
| 17β-dihydroequilin (40) |
110.75 ± 2.46 |
| Estrone (5000) |
128.84 ± 4.14** |
| Δ8,9-dehydroestrone (300) |
125.05 ± 1.42** |
Zhao and Brinton BMC Neuroscience 2006 7:24 doi:10.1186/1471-2202-7-24 |
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