Table 1

Plating parameters.

'Dense'

'Small'

'Sparse'

'Small & sparse'

'Ultra sparse'


Plating volume (μL)

20

5

20

5

20

Density of suspension (cells/μL)

2500

2500

625

625

156

Number of cells plated (nominal)

50,000

12,500

12,500

3,125

3,125

Culture diameter (mm)a

4.9 ± 0.4

3.1 ± 0.3

4.9 ± 0.4

3.1 ± 0.3

4.9 ± 0.4

Drop thickness (mm)a,b

1.69 ± 0.24

1.06 ± 0.23

1.69 ± 0.24

1.06 ± 0.23

1.69 ± 0.24

Density at 1 div (×103 cells/mm2)c

2.5 ± 1.5

1.6 ± 0.6

0.60 ± 0.24

0.30 ± 0.16

0.11 ± 0.06

Number of cultures followed

30

12

10

3

3

Number of batches

8

3

3

1

1


aMean ± sample standard deviation, N = 3 measured drops each of 5 and 20 μL.

bMeasured by focusing an inverted microscope on surface of MEA and top of drop (made visible by sprinkling some dust on it), and correcting for the refractive index of the liquid.

cMean ± sample standard deviation, based on N = 8, 8, 7, 3, 3 cultures. Cells in the central 0.36 mm2 of each MEA were counted using digital images.

Wagenaar et al. BMC Neuroscience 2006 7:11   doi:10.1186/1471-2202-7-11

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