Octopamine selectively enhances the pharmacologically separated fast inward current on B4 neuron. Ai. In the saline containing 50 mM tetraethylammonium (TEA) and 50 μM cadmium, the maintained outward current is reduced and the trace is dominated by a fast inward and transient outward current during five replicate depolarising voltage steps from -80 to -10 mV. (The small inward currents are caused by incomplete voltage clamp because of the high spontaneous activity of electrically coupled B4 cluster neurons). Aii. After 10 μM octopamine is added to this saline the fast inward component is increased without changing the outward component. Bi. In medium containing 50 mM TEA, 4 mM 4-aminopyridine (4-AP) and 50 μM cadmium,, and stepping from -50 to -20 mV, the outward components disappear leaving the fast inward component current (10 replicates). Bii. After adding 10 μM octopamine to the saline, the fast inward current is increased, and a smaller increase is seen in a sustained inward current.
Vehovszky et al. BMC Neuroscience 2005 6:70 doi:10.1186/1471-2202-6-70