Figure 8.

Leptin disrupts cortical actin filaments in GT1-7 cells Cultured GT1-7 cells were incubated in the absence and presence of leptin (10 nM) ± jasplakinolide (100 nM) for 30 minutes (jasplakinolide added 10 minutes prior to leptin). Following treatment cells were fixed and permeabilized, as described in the Methods, incubated with Alexa 488 conjugated phalloidin and Alexa 594 conjugated DNase I and subsequently processed for visualising F- and G-actin respectively by confocal microscopy.

Mirshamsi et al. BMC Neuroscience 2004 5:54   doi:10.1186/1471-2202-5-54
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