Foxo3a induces motoneuron death through the Fas pathway in cooperation with JNK

doi:10.1186/1471-2202-5-48

BMC Neuroscience

Volume 5

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Research article

Foxo3a induces motoneuron death through the Fas pathway in cooperation with JNK

Catherine Barthélémy , Christopher E Henderson and Brigitte Pettmann

UMR 623, Developmental Biology Institute of Marseille, Centre National de la Recherche Scientifique, Institut National de la Santé et de la Recherche Médicale, Université de la Méditerranée, Assistance Publique Marseille, Campus de Luminy-Case 907, 13288 Marseille, France

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BMC Neuroscience 2004, 5:48doi:10.1186/1471-2202-5-48


Published:	29 November 2004

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A – The 3 forms of Bim are expressed in motoneurons: RT-PCR was performed on extracts of 80,000 motoneurones cultured for 3 days in the presence of NTFs, in a 35 mm diameter dish. Primers used for Bim were the following: 5'-GTGACAGAGAAGGTGGACAAT-3' and 5'-ATACCAGACGGAAGATAAAGC-3'. The 3 products were:BimS 284 bp, BimSL 374 bp, BimL 542 bp; B – Overexpression of Bim_Sor Bim_Lkills a major proportion of motoneurons: motoneurons purified from E12.5 mice embryos have been electroporated with a vector coding GFP alone or coelectroporated with a vector coding GFP and a vector coding either BimL or BimS, obtained by direct cloning of the PCR products described above first cloned into pGEMTeasy, then subcloned into pcDNA3 into EcoRI sites. Conditions of electroporation were as described in Material and Methods. The surviving electroporated motoneurons were counted after 2 days in culture in the presence of NTFs.

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