Specificity of increased antibody binding after acid-dissociation of sera from Aβ immunized APP transgenic mice. Sera were collected from transgenic mice, diluted 1:400 and preincubated at either pH 7 (open bars) or pH 2.5 (solid bars) as described in methods. The sera were then incubated on ELISA plates coated with Aβ1-42, phosphate buffered saline (PBS; a "no protein" control), bovine serum albumin (BSA), recombinant alpha-synuclein (α-SYN) or Aβ peptide amino acids 11–20. ELISA assays were completed as described in Methods and the optical density at 450 nm used to estimate the amount of antibody binding to the ELISA plate. Results are presented as mean ± sem.
Li et al. BMC Neuroscience 2004 5:21 doi:10.1186/1471-2202-5-21