Figure 5.

Effect of Smad inhibition on the TGF-β1 activation of Ant1. Primary astrocytes were transiently co-transfected with the TGF-β1 responsive Ant1 485 bp reporter construct and with expression constructs for the indicated dominant negative Smads or empty vector control. Levels of Ant1 promoter activity induction by TGF-β1 in the presence of individual dominant negative Smads and empty vector control were assessed by one-way ANOVA (p < 0.05). Individual dominant negative Smad treatments were compared relative to empty vector control by Dunnett's post hoc multiple comparison test.