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A new way to rapidly create functional, fluorescent fusion proteins: random insertion of GFP with an in vitro transposition reaction

Douglas L Sheridan1, Catherine H Berlot2, Antoine Robert3, Fiona M Inglis3, Klara B Jakobsdottir4, James R Howe3 and Thomas E Hughes4*

Author Affiliations

1 Interdepartmental Neuroscience Program, Yale University Medical School, 330 Cedar St, New Haven, CT 06520, USA

2 Department of Cellular and Molecular Physiology, Yale University Medical School, 330 Cedar St, New Haven, CT 06520, USA

3 Department of Pharmacology, Yale University Medical School, 330 Cedar St, New Haven, CT 06520, USA

4 Department of Ophthalmology & Visual Science, Yale University Medical School, 330 Cedar St, New Haven, CT 06520, USA

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BMC Neuroscience 2002, 3:7  doi:10.1186/1471-2202-3-7

Published: 19 June 2002

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