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|
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| Antigen |
Primary |
Dilution |
Antibody |
Secondary |
Specificity & |
| antibodies/ |
Source |
antibodies/antisera |
Subcellular distribution |
||
| antisera |
(dilutions) |
||||
|
|
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| Estrogen |
Mouse |
1:10 |
Chemicon |
Goat anti-mouse IgG |
Neurons and astrocytes / |
| receptor |
IgG1 |
(MAB428) |
(H+L)conjugated to Cy3 |
Punctate nuclear and |
|
| (ER) |
monoclonal |
(1:250) |
perinuclear distribution |
||
| Dopamine |
Rabbit |
1:1000 |
Chemicon |
• Goat anti-rabbit IgG |
Neuronal processes and cell |
| (DA) |
IgG |
(AB122S) |
DTAF (1:500) or |
body |
|
| polyclonal |
• Goat anti-rabbit IgG |
Cytoplasmic distribution |
|||
| Cy3 (1:250) |
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| Dopamine |
Rat |
1:100 |
Chemicon |
Goat anti-rat IgG |
Neuronal processes and cell |
| transporter |
Ig type |
(MAB369) |
conjugated to biotin- |
body; astrocytes |
|
| (DAT) |
monoclonal |
avidin-Cy2 (1:250) |
Cell membrane |
||
| Dopamine |
Rabbit |
1:100 |
Chemicon |
Goat anti-rabbit IgG |
Neurons and astrocytes; |
| D1A |
polyclonal |
(AB1784P) |
DTAF (1:500) |
punctate distribution |
|
| receptor |
|||||
| Dopamine |
Rabbit |
1:500 |
Chemicon |
Goat anti rabbit IgG |
Neuronal processes and cell |
| D2 |
polyclonal |
(AB1558) |
DTAF (1:500) |
bodies: perinuclear |
|
| receptor |
distribution, astrocytes, |
||||
| microglia |
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|
|
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| Co-localization |
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|
|
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| Combined |
• Goat anti-rabbit IgG |
Colocalized in neurons |
|||
| • DA + |
as above |
as above |
as above |
DTAF + |
and/or astrocytes |
| • ER |
• Goat anti-mouse |
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| Cy3 |
|||||
| Combined |
• Goat anti-rabbit IgG |
Colocalized in |
|||
| • DA + |
as above |
as above |
as above |
Cy3 |
subpopulations of neurons |
| • DAT |
• Goat anti-rat IgG |
and/or astrocytes |
|||
| biotin-avidin Cy2 |
|||||
| Combined |
as above |
as above |
as above |
• Goat anti-rabbit IgG |
Colocalized in |
| • D1A + |
DTAF (1:500) |
subpopulations of neurons |
|||
| • ER |
• Goat anti-mouse |
and/or astrocytes |
|||
| Cy3 (1:250) |
|||||
| Combined |
as above |
as above |
as above |
• Goat anti-rabbit IgG |
Colocalized in |
| • D2 + |
DTAF (1:500) |
subpopulations of neurons |
|||
| • ER |
• Goat anti-mouse |
and/or astrocytes and |
|||
| Cy3 (1:250) |
microglia |
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Turchan et al. BMC Neuroscience 2001 2:3 doi:10.1186/1471-2202-2-3 |
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