Quantitation of necrotic cells under hydrostatic pressure and calpain inhibition. Panel A: Normal control cells; Panel B: RGC-5 cell lines necrosis under elevate hydrostatic pressure; Panel C: RGC-5 cell lines were pre-treated with ALLN to inhibit calpain activity for 24 hr before elevating hydrostatic pressure; Panel D: The statistical analysis of RGC-5 necrosis. Cells were stained with Annexin FITC and PI, and analyzed by FACS using FL1 (Annexin) and FL3 (PI) channels. *: 24 hr group vs CTL or 24 hr-ALLN treated group: p < 0.05.
Shang et al. BMC Neuroscience 2014 15:63 doi:10.1186/1471-2202-15-63