Plots of Log2 Fold Change for analysed genes, following single neurotrophin treatments. (A) Plots of Log2 Fold Change (FC) for the analysed genes, at 5, 15, 30, 60 minutes, with the two treatments: proNGF/NGF = 0/10 ng/ml and proNGF/NGF = 20/0 ng/ml. At 5 and 15 minutes, a third treatment with high proNGF is shown, proNGF/NGF = 200/0 ng/ml. The Group I genes show a stronger proNGF-mediated effect at 15 minutes with proNGF/NGF =200/0 and at 30 minutes with proNGF/NGF =20/0. (B) Average Log2 Fold Change ratio in the two groups of genes shown in (A). The proNGF/NGF =200/0 and proNGF/NGF =20/0 treatments originate a similar response, with a significant increase in gene transcription at 15 and 30 minutes, respectively: this effect is even more significant for the genes of group I highlighted in Figure 2A. (*****) 1-tail T-test with unequal variance, p < 0.00001. (***) 1-tail T-test with unequal variance, p < 0.001. (*) 1-tail T-test with unequal variance, p < 0.05. Error bars are s.e.m.
Arisi et al. BMC Neuroscience 2014 15:48 doi:10.1186/1471-2202-15-48