Quantitation of viral transduction depicted in Figure6. In this experiment, AAV-RFP virus was pseudotyped as AAV2/1, 2/5, 2/7, 2/8, 2/9, 2/rh10, 2/DJ, or 2/DJ8 and infused into the BLA at the titer of 7.8E + 11 GC/ml. (A). Viral transduction (OD * area (μm2)) was quantified and depicted using methods described in Figure 5. (B). Viral OD was quantified and depicted using methods described in Figure 5. (C). Viral spread (area (μm2)), was quantified and depicted using methods described in Figure 5. (D). The number of transduced cells was quantified for each viral infusion and these data were averaged across infusions and reported as mean number of transduced cells. These measurements included measuring the number of transduced cells for the entire viral transduction (Total) and the number of transduced cells for the viral transduction that were confined to the BLA (BLA only). (E). For analysis of viral transduction across the anterior-posterior axis of the BLA, the amygdala was subdivided into 5 regions with respect to Bregma: -2.3 (-1.8 - -2.7); -2.8 (-2.7 - -3.1); -3.3 (-3.1 - -3.4); -3.6 (-3.4 - -3.7); 3.8 (-3.7 - -4.0) (see Figure 3) and the total BLA only transduction was quantified within each subregion. Graphs displayed in A. C. D. and E. have logarithmic scales. Error bars represent the standard error of the mean.
Holehonnur et al. BMC Neuroscience 2014 15:28 doi:10.1186/1471-2202-15-28