Representative coronal images containing the amygdala (~ Bregma -3.3) depicting AAV-RFP viral transduction for selected AAV serotypes (RFP fluorescence) and associated bright field images. In this experiment, AAV-RFP virus was pseudotyped as AAV2/1, 2/2, 2/5, 2/7, 2/8, 2/9, 2/rh10, 2/DJ, or 2/DJ8 and infused into the BLA at the highest titer achieved for each respective serotype (see Figure 2C). Images of coronal brain sections containing the amygdala are arranged for each serotype as follows: bright field image (scale bar = 500 μm), RFP image, and magnified view of a portion of the BLA region of the adjacent RFP image to allow visualization of the cellular detail (scale bar = 100 μm). LA = lateral nucleus of the amygdala, B = basal nucleus of the amygdala. For publication purposes, RFP images for AAV1, 5, 7, and 8 were slightly enhanced to increase clarity. AAV2/2 and AAV2/2* are the same serotype (see Methods) and neither of these viruses transduced any cells within the BLA at the respective titers achieved.
Holehonnur et al. BMC Neuroscience 2014 15:28 doi:10.1186/1471-2202-15-28