Halting the progression of neurodegeneration by Ubisol – Q10. (A) The experimental plan demonstrating the injection regime and treatment schedule. Immunohistochemistry performed using anti-tyrosine hydroxylase antibodies on the brain sections of animals. (B) Representative images of midbrain sections showing TH positive neurons at lower and higher magnifications from saline injected control group rats, PQ injected rats fed with regular drinking water dissected immediately after the last injection (PQ1), PQ injected rats fed with regular drinking dissected 4 weeks after last injection (PQ2), PQ injected rats fed with Ubisol – Q10 supplemented drinking water after last injection and dissected 4 weeks after last injection (PQ2 + Ubisol- Q10 4 weeks). The area of SN which is to be counted is selected in every 6th section of the midbrain (sectioned at 30 microns thickness). The total number of TH positive neurons in the SNpc region was counted at high magnification using the stereology software purchased from the Stereology Resource Centre, Inc., Florida. (C) The percentage decrease in TH positive neurons between the saline injected control group and the PQ injected treated and untreated groups. There is a significant 41% decrease in the TH positive neurons in the PQ2 group in comparison with the saline injected control group (*p < 0.05), whereas there is loss of 17% neurons in the Ubisol – Q10 treated group verses the control (**p < 0.05) indicating significant neuroprotection when compared to the PQ2 group. The bars in the upper (low magnification) panel are 200 μm and in the lower (high magnification) panel 20 μm.
Muthukumaran et al. BMC Neuroscience 2014 15:21 doi:10.1186/1471-2202-15-21