Figure 3.

Increased ROS levels in response to UVC irradiation. Confocal microscope images of w1118 dissected larval body walls that were incubated with 10 μM carboxyl-H2DCFDA prior to UVC irradiation. (AB) Larval body wall images at epidermal focal plane. (A) Control wild-type (w[1118]) larvae showed no detectable fluorescence in the absence of UVC irradiation. (B) UVC-irradiated larval body walls displayed a significant increase in fluorescence in epidermal tissues indicating production of ROS in response to irradiation. (CD) Larval body wall images at muscle layer focal plane. (C) Wild-type controls showed no detectable fluorescence in the absence of UVC irradiation. (D) UVC-irradiated larval body walls displayed a significant increase in fluorescence in muscle layers indicating production of ROS in response to irradiation.

Kim and Johnson BMC Neuroscience 2014 15:14   doi:10.1186/1471-2202-15-14
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