New Olig1 null mice confirm a non-essential role for Olig1 in oligodendrocyte development
1 Wolfson Institute for Biomedical Research and Research Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK
2 Present address: Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal
BMC Neuroscience 2014, 15:12 doi:10.1186/1471-2202-15-12Published: 14 January 2014
Additional file 1: Figure S1:
No NICD expression in Olig1(+/-) or Olig1(-/-) mice. Proteins from E18.5 spinal cord were subjected to SDS-PAGE, followed by Western blotting with rabbit anti-Myc antibody. pPGKcreSV40-transfected MEFs derived from Olig1(+/-) embryos were used as positive control. The NICD band is indicated by an arrow.
Format: JPEG Size: 116KB Download file
Additional file 2: Figure S2:
Our new Olig1 null mice do not express Olig1 protein. Co-immunolabeling for Olig1 (green) and Sox10 (red) was performed on sections of E18.5 mouse spinal cords. No Olig1-positive cells were detected in either Olig1(-/-) (B, B’) or Olig(-/-),Olig2(Tg) spinal cords (D, D′) in contrast to in Olig1(+/-) (A, A’) or Olig(+/-),Olig2(Tg) controls (C, C′). Scale bar: 80 μm for A-D and 20 μm for A’-D’.
Format: JPEG Size: 1.5MB Download file
Additional file 3: Figure S3:
No up-regulation of Olig2 expression in Olig1(-/-) spinal cord. Quantitative PCR using cDNA templates prepared from E18.5 spinal cord tissue revealed that there was no appreciable difference in the expression of Olig2 mRNA between Olig1(+/+) and Olig1(-/-) lines or between Olig(+/-),Olig2(Tg) and Olig(-/-),Olig2(Tg) lines.
Format: JPEG Size: 125KB Download file
Additional file 4: Figure S4:
No change in OL numbers in Olig1 null forebrain at P7. In the developing forebrain, OL differentiation starts in the first postnatal week. At P7, coronal sections showed that the numbers of Mbp- and Plp-expressing cells in Olig1 null forebrain (B,F and D,H respectively) were similar to those in controls (A,E and C,G respectively). cc, corpus callosum; ctx, cortex. Scale bar: 80 μm.
Format: JPEG Size: 1.4MB Download file