Figure 3.

Loss of Whirlin contributes to abnormal paranodal compaction and cytoskeleton instability throughout sciatic nerve age. AI. 2-week, 10, 20, 30, 40-week, and 1-yr-old teased sciatic nerve fibers either wild-type (AaAh, CaCh, HaHh) or Whrn knockout (BaBh, DaDh, EaEh, FaFh, GaGh, IaIh) immunostained against Kv1.2 (Aa-Ia, red), Caspr (Ab,fIb,f, green), NF186 (Ac-Ic, blue), heavy chain Neurofilament/Nfl-H (Ae-Ie, red), Protein 4.1 band/4.1B (Ag-Ig, blue), and merged images (Ad,hHd,h). In all Whrn mutant panels, Caspr (Bb,d,f,h; Db,d,f,h; Eb,d,f,h; Fb,d,f,h; Gb,d,f,h, Ib,d,f,h, green) fail to compact properly at the paranodes while juxtaparanodal marker Kv1.2 (Ba,Bd; Da,Dd; Ea,Ed; Fa,Fd; Ga,Gd; Ia,d, red) appears diffuse and disorganized. Nodal NF186 (Ac-Ic, blue) appear unaffected by genotype or age. Cytoskeletal markers like Nfl-H (De-Ge vs. Ce, Ie vs. He, red) and 4.1B (Dg-Gg vs. Cg, Ig vs. Hg, blue) show more diffusion and irregularities in intensity, particularly older animals, in Whrn mutant nerves compared to wild-type nerves, suggesting Whrn contributes to paranodal cytoskeletal stability. Scale bars (Ad-Id, Ah-Ih) = 5 μm. Further quantification and percentages of phenotype-positive sciatic nerves is included for each age/genotype in a summary graph (bottom).

Green et al. BMC Neuroscience 2013 14:96   doi:10.1186/1471-2202-14-96
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