Coexposure to the MEK and the PKA inhibitors reduces DOM-stimulated DCX upregulation. (A) Organotypic slices were cultured in the presence of the MEK inhibitor PD98059 or the PKA inhibitor H89 for 1 h, and then treated with 2 μM DOM for 24 h (insult). Lysates were collected at the indicated hours post insult (HPI). Compared to DOM alone, the increased DCX levels were significantly reduced when DOM was co-incubated with PD98059. However, H89 failed to decrease DCX up-regulation. The blots correspond to representative experiments and values are the means ± SEM of at least three experiments performed from different cultures. (B) Organotypic cultures were treated with PD98059 and H89 for 1 h, and then exposed to 2 μM DOM for 24 h. Lysates were collected at the indicated HPI and analyzed by Western blot. The blots correspond to representative experiments and values are the means ± SEM of at least four experiments performed from different cultures. (#P < 0.001 vs. None within the same group; *P < 0.02 vs None within C group).
Pérez-Gómez and Tasker BMC Neuroscience 2013 14:72 doi:10.1186/1471-2202-14-72