Transient DOM exposure induces ERK, PKA and CaMKII activation in OHSC. Cultures were treated with 2 μM DOM for 24 h (insult) and then changed to a DOM-free medium; lysates were collected at different hours post-insult (HPI) and analyzed by Western Blots for the active, phosphorylated form of ERK (A, p-ERK, PKA) (B, p-PKA) or CaMKII (C, p-CaMKII). The blots were then reprobed for total ERK, PKA or CaMKII protein and finally for β-actin. The blots correspond to representative experiments and values are the means ± SEM of at least three experiments performed from different cultures (*P < 0.01 vs. C).
Pérez-Gómez and Tasker BMC Neuroscience 2013 14:72 doi:10.1186/1471-2202-14-72