Immunohistochemical visualization of BDNF in microglia, neurons and astroglial cells in CA1 area, following excitotoxic insult. (A) Representative fluorescence photomicrographs of CD11b-positive microglial cells (red), BDNF (green), DAPI and merge images. Upper row: control culture, in which BDNF-positive microglial cells have resting-like morphology. Lower row: culture exposed to DOM for 24 h and then transferred to a DOM-free medium for 7 days showed highly activated and BDNF-expressing microglia (lower left quadrant). (B) Representative fluorescence photomicrographs of NeuN-positive neurons (red), BDNF (green), DAPI and merge images. Most BDNF immunoreactivity in both control and DOM-treated group was observed in NeuN-positive cells. (C) Representative photomicrographs of GFAP-positive astroglial cells (red), BDNF (green), DAPI and merge images. No significant changes were observed in the number of astrocytes expressing BDNF in presence or absence of the DOM insult.
Pérez-Gómez and Tasker BMC Neuroscience 2013 14:72 doi:10.1186/1471-2202-14-72