Ganglion cell dendrites express little, if any, P-Rex2. A-C: Confocal imaging of P-Rex2 and Microtubule-Associated Protein-1 (MAP-1) labeling in the IPL. A: P-Rex2 labeling is present in discrete puncta located throughout the IPL. B: Labeling for MAP-1 is present in ganglion cell dendrites throughout the IPL, as well as ganglion cell bodies in the GCL and their axons (Ax). C: Overlay of panels A and B shows that P-Rex2 and MAP-1 are independently distributed. Nuclei are counterstained with DAPI in panel A to illustrate the location of the INL and GCL. Single optical plane of approximately 0.3 μm thickness shown. IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bars = 20 μm.
Sherry and Blackburn BMC Neuroscience 2013 14:70 doi:10.1186/1471-2202-14-70