Open Access Highly Accessed Research article

LOC689986, a unique gene showing specific expression in restricted areas of the rodent neocortex

Kari M Ersland123*, Bjarte Håvik123, Johanne Egge Rinholm4, Vidar Gundersen45, Christine Stansberg123 and Vidar M Steen123

Author Affiliations

1 Dr E. Martens Research Group for Biological Psychiatry, Department of Clinical Science, University of Bergen, Bergen, Norway

2 Center for Medical Genetics and Molecular Medicine, Laboratory Building, Haukeland University Hospital, Bergen, Norway

3 KG Jebsen Centre for Psychosis Research, Oslo, Norway

4 Department of Anatomy and Centre for Molecular Biology and Neuroscience, University of Oslo, Oslo, Norway

5 Department of Neurology, Oslo University Hospital, Oslo, Norway

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BMC Neuroscience 2013, 14:68  doi:10.1186/1471-2202-14-68

Published: 11 July 2013

Additional files

Additional file 1:

Cortical tissue dissection. Consecutive side-by-side tissue samples were extracted from the parietal- temporal- and occipital lobe from the adult rat brain. Tissue samples, covering a matrix of 25 samples, from a total of 6 individual rats were analysed (numbered 1-25). The uppermost row (sample number 21-25) represents samples from the cingulate cortex (arrows indicates the direction). The figure is based upon an image of the adult rat brain (lateral view, image courtesy of Adam C. Puche), acquired from The Olfactory Image Archive.

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Additional file 2:

LOC689986 gene expression in the rat neocortex. qRT-PCR analysis of the relative LOC689986 gene expression level in corresponding cortical samples from 6 rats. The relative gene expression level was normalised to the endogenous control Actb. Standard error of the mean is indicated for all the samples. x-axis: samples (corresponding to the areas shown in Additional file 1), y-axis: average relative gene expression level, *: samples from five individual rats.

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Additional file 3:

In situ RNA hybridisation analysis of LOC689986 gene expression in the brain. The LOC689986 gene expression was analysed in representative coronal sections from the whole adult rat brain (20 μm floating sections). The strongest gene expression was observed in layer 4 of the somatosensory cortex. A. Bregma 0.48 mm, B. Bregma -0.24 mm, C. Bregma -1.72. The areas shown in A, B and C correspond to regions illustrated in the schematic drawing of the adult rat brain (drawing was obtained from motifolio.co).

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Additional file 4:

Expression pattern of the human orthologous gene C1orf146. qRT-PCR analysis of the relative C1orf146 gene expression level in tissue samples from 8 different human brain regions (x-axis). The gene expression of C1orf146 was normalised against the endogenous control ARBP. The relative gene expression level is indicated on the y-axis. Ct values are listed below.

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Additional file 5:

Microarray gene expression pattern of C1orf146 in 32 different human tissue samples. The samples are from the Tissue Gene Expression Database (Human Body Map, Applied Biosystems), and are listed on the x-axis. y-axis: normalised signal intensity.

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Additional file 6:

LOC689986 is located in astrocytes. Confocal laser scanning images of a section from piriform cortex that was double labelled for LOC689986 (A, green) and the astrocytic marker glutamine synthetase (B, purple). The overlay (C) shows that astrocytes are labelled for LOC689986 (white). Arrows in A and B highlight LOC689986 positive astrocytes. The inset shows a double labelled astrocyte at higher magnification.

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