Withdrawal behavior is dependent on the ramp rate of the thermal stimulus. The mean speed profile of wild type animals for three head-applied stimuli with the same ramp rate but different absolute temperatures (N = 10 for each ∆T), showing a noxious response for each. Animals stimulated with a lower ramp rate but similar ∆T do not exhibit withdrawal behavior, and are statistically different than those stimulated with a higher ramp rate. p < 0.05, Kruskal-Wallis test, Dunn’s multiple comparison.
Mohammadi et al. BMC Neuroscience 2013 14:66 doi:10.1186/1471-2202-14-66