RT-qPCR validation of micro-array results in brain regions. A-C show RT-qPCR validation results in prefrontal cortex (PFC), caudate-putamen (CPU), and hippocampus (HIPPO) represented as mean + SEM normalized to control (n = 4-6). Leptin reveals a significant difference in CPU and HIPPO (p < 0.05), Rdh2 demonstrates only a significant difference in HIPPO (p < 0.05), and Smad6 does not show significant differences in the investigated brain regions. (White bars = Control, grey bars = Fetal Asphyctic Preconditioning, significance was tested with unpaired two-tailed Student’s t-test: * = p < 0.05).
Cox-Limpens et al. BMC Neuroscience 2013 14:61 doi:10.1186/1471-2202-14-61