Characterisation of BBB protein expression in cultured RBECs by immunocytochemistry. Images show fixed and permeabilised RBECs cells grown on collagen I and fibronectin-coated 96-well plates and stained with antibodies for (a) von Willebrand Factor, (b) smooth muscle actin, (c) claudin-5, (d) ZO-1, (e) occludin, (f) clathrin heavy chain, (g) caveolin-1, (h) P-gp. Images are representative of 3 independent cultures, with five fields of view taken from each individual preparation of cells using the 20× objective [10× for 5 (b) for a wider field of view] on an Olympus IX81 microscope.
Watson et al. BMC Neuroscience 2013 14:59 doi:10.1186/1471-2202-14-59