Additional file 4: Figure S3.
Wild-type males, females, and fru mutant males have similar neurotransmitter profiles for brain and VNC-T1midline neurons, but vary for VNC-medial neurons in T1, T2, and T3. CNSs of wild-type males (A, C, F, G, H), wild-type females (B, D, I), and fru-mutant males (E, J) were labeled for anti-FRUM (green) and anti-Engrailed (magenta), and a neurochemical marker (blue). (A, B) E/F-VNCmid neurons in males, and the equivalent in females, express gamma-Aminobutyric acid (GABA) neurotransmitter as labeled by anti-GABA antibody. (C, D, E) In males, females, and fru mutant males E/F-VNCmid neurons are also labeled by anti-GAD antibody (GAD = Glutamic Acid Decarboxylase, an enzyme for GABA synthesis, localized exclusively to GABAergic neurons). (F, G) E/F-brain (F) and E/F-AbG (G) neurons are also labeled by GAD in wild-type males. (H, I, J) Males expressed a Ddc-GAL4; UAS-mcd8::GFP in E/F-VNCmed neurons of T1, T2 (H) and T3 (not shown). Females (I) and fru-mutant males (J) express this driver much more faintly, in with variable penetrance in the equivalent neurons. (Ddc = dopa Decarboxylase, an enzyme for serotonin/5HTsynthesis). (K, L, M) Schematic indicating neurotransmitter profile of En/FRUM neurons in the brain (K), T1/T2 segments of the VNC (L), and T3 and AbG (M).
Format: DOCX Size: 16KB Download file
Latham et al. BMC Neuroscience 2013 14:57 doi:10.1186/1471-2202-14-57