Expression of Mcl1 in brains from rats subjected to middle cerebral artery occlusion (MCAO). (A) A schematic representation of a coronal brain section. The square fields represent observed regions (a). Immunofluorescent staining showed the expression of Mcl1 in brains from sham-operated control (b) and MCAO group (4 h, 24 h, 72 h, c-e). Quantification of Mcl1-positive cells (f). Results were expressed as mean ± SD from four independent experiments. *P < 0.001 vs. control group, #P < 0.001 vs. the previous time point. Scale bars = 50 μm. (B) The co-localization of Mcl1 and DAPI indicated Mcl1was mainly localized in the cytoplasm. Scale bars = 50μm. (C) Western blot confirmed that Mcl1 protein levels were elevated in MCAO group at each time point compared with control group. Optical densities of respective protein bands were analyzed with Image J 1.42q and normalized to the loading control (β-actin). Results were expressed as mean ± SD from four independent experiments. *P < 0.001 vs. control group, #P < 0.001 vs. the previous time point.
Xingyong et al. BMC Neuroscience 2013 14:56 doi:10.1186/1471-2202-14-56