GFAP immunoreactivity 3 or 7 days after a single injection in the CeA. A. 3 days after a single saline injection. B. 7 days after a single saline injection. C. 3 days after a single injection of AONs. D. 7 days after a single injection of AONs. E. GFAP immunoreactivity in the CeA of an untreated mouse. F. Quantification of GFAP immunoreactive area shown as percentage of the total area of visual field. One-way ANOVA followed by Tukey’s post hoc test revealed no significant differences between the respective AON and saline injected animals (one-way ANOVA F(4,17)=1.266, p>0.32, N=3-7 animals per group). In conclusion, a single AON injection did not induce stronger astrocytosis than saline. Scale bar 50 μm. Red: GFAP, blue: Hoechst. Green signal (AONs) has been omitted for clarity.
Zalachoras et al. BMC Neuroscience 2013 14:5 doi:10.1186/1471-2202-14-5