Effect of 10 μM RA on the expression of functional voltage-sensitive. Ca2+ channels in M17 cells. M17 cells were treated with 1 mCi/ml 3H-Valine 24 hours prior to each experiment. M17 cells were either (A) undifferentiated cells stimulated for 4 minutes with KCL, or differentiated cells (B) stimulated for 4 minutes with KCl, (C) KCl + 10 μM NNC 55-0396/KCl, (D) KCl + 1 mM w conotoxin, GVIA, or (E) KCl + 300 nM w agatoxin IVA. Each of these KCl solutions contained 1 mCi/ml of 45Ca2+. The ratio of 45Ca2+/3H was then used to calculate the percentage difference of Ca2+ channel activity. % of control was calculated by dividing the experimental ratio of 45Ca2+/3H by the ratio of 45Ca2+/3H generated with 5.9 mM KCl alone. n=4 *p<0.05 when compared to 5.9 mM KCl. **p<0.01 when compared to 5.9 mM KCl.
Andres et al. BMC Neuroscience 2013 14:49 doi:10.1186/1471-2202-14-49