Expression of Neuron Specific Proteins in M17 cell cultures. M17 neuroblastoma cells were grown and treated with or without 10 μM RA for 72 hours to induce differentiation. The cells were washed and solubilized in sample buffer and analyzed by Western blotting for (A) SNAP-25, (B) synapsin, and (C) vimentin. Either β-actin or GAPDH was used as a house keeping protein marker to show equal protein loading of gels. The relative amount of each marker protein was quantified by densitometric analysis using Image J program (NIH public domain program, http://rsbweb.nih.gov/ij/index.html webcite). Differences in marker proteins in differentiated vs. undifferentiated cells were assessed in terms of % normalized optical density in differentiated cells compared to undifferentiated (no RA) controls as shown in the bottom panel (D). Normalized optical density of undifferentiated control was 100% for each type of marker protein. **p<0.01, *p<0.05, using the student t test. n=6.
Andres et al. BMC Neuroscience 2013 14:49 doi:10.1186/1471-2202-14-49