Effect of chondroitin 4-sulfate (CS) incorporated to nECM on hippocampal cell viability. (A) Experimental set up. After one week on CS-containing nECM and 24 hours of stressor treatment, hippocampus cell viability was measured by MTT assay. (B) Percentage of viability of hippocampal cells cultured on nECM in the absence (black bar) or presence of CS (1.5 mg/ml, grey bar; 15 mg/ml, white bar). (C) Percentage of viability of hippocampal cells cultured on nECM in the absence/presence of CS and in the absence/presence of 100 μM glutamate treatment. In each case, the treatments applied are described below bars. (D-E) Same as previous but this time in the presence of 3 μM amyloid beta 23:35 peptide (Aβ) (D) and 1 μM oligomycin plus 3 μM rotenone (O/R) (E) for 24 h. ** 0.001 < P < 0.01. The numbers between brackets on top of panels B-D show the number of independent experiments performed in each case. (F-G) Composite image containing immunofluorescence images of cells cultured on control nECM (F) and on 15 mg/ml CS-containing nECM (G). Cells were immunolabelled with anti-MAP2 antibody (green) and nuclei counterstained with Hoechst (blue).
García-Parra et al. BMC Neuroscience 2013 14:48 doi:10.1186/1471-2202-14-48