Quantitative assessment of hippocampal neuronal and glial differentiation on PLL- and nECM-coated coverslips. (A-C) To estimate the total cell number in each photograph, the number of nuclei stained by Hoechst was counted (A) and the percent of cells positive for MAP2 (B) and GFAP (C) immunostains were determined. (D-F) Focusing on MAP2 positive cells, the number of cells sprouting neurites (D) as well as the total number of neurites (E) was determined at 24 and 72 h, respectively. Next, the number of neurites per neuron was calculated (F). At least six different fields out of three independent experiments were quantified at each substrate and time point. Statistical analyses were done by using two-way ANOVA followed by Bonferroni’s posttests (*P < 0.05; ** P < 0.01; *** P < 0.001).
García-Parra et al. BMC Neuroscience 2013 14:48 doi:10.1186/1471-2202-14-48