Open Access Highly Accessed Methodology article

A neural extracellular matrix-based method for in vitro hippocampal neuron culture and dopaminergic differentiation of neural stem cells

Patricia García-Parra12*, Marcos Maroto3, Fabio Cavaliere4, Neia Naldaiz-Gastesi12, José Iñaki Álava5, Antonio G García3, Adolfo López de Munain2 and Ander Izeta1

Author Affiliations

1 Tissue Engineering Laboratory, Department of Bioengineering, Instituto Biodonostia, Hospital Universitario Donostia, San Sebastian, 20014, Spain

2 Neuroscience Area and CIBERNED, Instituto Biodonostia, Hospital Universitario Donostia, San Sebastian, 20014, Spain

3 Instituto Teófilo Hernando de I + D del Medicamento. Departamento de Farmacología y Terapeútica and Servicio de Farmacología Clínica del IIS Hospital Universitario de La Princesa, Facultad de Medicina, Universidad Autónoma de Madrid (UAM), Madrid, 28029, Spain

4 Department of Neuroscience and CIBERNED, University of Basque Country (UPV/EHU), Zamudio, 48170, Spain

5 Basque Culinary Center R&D, San Sebastian, 20009, Spain

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BMC Neuroscience 2013, 14:48  doi:10.1186/1471-2202-14-48

Published: 18 April 2013

Additional files

Additional file 1: Video S1:

Three-dimensional analysis of SVZ-derived progenitors differentiated on PLL-coated substrates for 10 days. A 3D projection of 40 consecutive confocal sections obtained from PLL-coated coverslips is shown. The depth of imaging was 14.6 microns with a thickness (interval) of 0.374 microns between sections. Video edition was done with ZEN 2008 and ImageJ software. Magnification: 480X.

Format: AVI Size: 4.8MB Download file

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Additional file 2: Video S2:

Three-dimensional analysis of SVZ-derived progenitors differentiated on nECM-coated substrates for 10 days. A 3D projection of 40 consecutive confocal sections obtained from nECM-coated coverslips is shown. The depth of imaging was 14.98 microns with a thickness (interval) of 0.384 microns between sections. Video edition was done with ZEN 2008 and ImageJ software. Magnification: 480X.

Format: AVI Size: 6.4MB Download file

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Open Data