Additional file 2: Figure S1.
Representative example of SDS-PAGE fractionation (4-12% Bis-Tris Midi gradient gel) and immunoblot analysis (primary antibody: 6E10 mAb, 1:500; secondary antibody: goat anti-mouse, IrDye 680-labeled antibody, 1:3000) carried out on “low-detergent”, 0.01% NP40/0.1% SDS brain extracts (enriched in extracellular Aβ). Immune-reactive bands were visualized by near-infrared fluorescence (Odyssey imager, LI-COR). Non-specific, 6E10 mAb cross-reactive polypeptides (arrow), present in both wild-type and Tg2576 brain extracts, were used as loading controls and internal references for data normalization. Synthetic prefibrillar Aβ42(n) prepared according to Lambert et al. , with n-values ranging from 1 to 4 (not shown), was used as size standard for electrophoretic analysis. Immune-reactive bands were quantified as “near-infrared fluorescence” (NIRF) arbitrary units (see ‘Methods’ for additional details).
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Sivilia et al. BMC Neuroscience 2013 14:44 doi:10.1186/1471-2202-14-44