Quantitative examination of WDR35 expression in rat hippocampus following DA administration. (A, B) DA was administered to rats intraperitoneally at various doses. (A) WDR35 mRNA expression in hippocampal RNA preparations at 24 hours after DA administration was analyzed by qRT-PCR and expressed relative to the expression of GAPDH mRNA. (B) WDR35 protein expression in hippocampal homogenates at 24 hours after DA administration was analyzed by western blotting. (C, D) DA (1 mg/kg) was administered to rats intraperitoneally and WDR35 mRNA expression (C) and WDR35 protein expression (D) were determined in hippocampal samples collected at the times indicated after DA administration using the same methods as were used in (A) and (B). *P < 0.05, **P < 0.01 and ***P < 0.001 (n = 4).
Tsunekawa et al. BMC Neuroscience 2013 14:4 doi:10.1186/1471-2202-14-4