The proliferation and viability of NSCs in high glucose were analyzed using the EdU incorporation assay and CCK-8 assay. (a) EdU-labeled cells appear in purple as the EdU (red) is colocalized with DAPI (blue). The scale bar represents 20 μm. (b) Note the decreased number of EdU-positive cells in NSCs exposed to high glucose (41.75 or 83.75 mM), indicating less proliferation. However, there was no difference in EdU incorporation between the 27.75 mM glucose treatment and control. % of EdU/DAPI means percentage of EdU-positive cells in NSCs. *P < 0.01 versus control, analyzed by one-way ANOVA/Bonferroni post hoc test versus the normal glucose group (17.5 mM glucose). (c) High glucose exposure caused a significant decrease in the viability of NSCs following OGD. *P < 0.01 versus control, analyzed by one-way ANOVA/Bonferroni post hoc test.
Chen et al. BMC Neuroscience 2013 14:24 doi:10.1186/1471-2202-14-24